Pol II ChIP-Seq Service
Alternate approach to RNA-based gene expression methods
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Active Motif’s TranscriptionPath™ Assay is an alternate approach to RNA-based gene expression methods.
The TranscriptionPath method utilizes Chromatin Immunoprecipitation (ChIP) to measure transcription rates as a function of RNA Polymerase II (RNAPII) occupancy across the genome. The assay can be combined with Next-Generation sequencing (TranscriptionPath-Seq) for a genome-wide readout, or it can be used with qPCR for a gene-specific readout. TranscriptionPath offers several advantages when compared to RNA-based approaches including the ability to look at early time points that are not accurately measured with mRNA approaches and the ability to measure gene expression in the same sample as was used to measure transcription factor (TF) binding by ChIP.
Advantages of using TranscriptionPath to measure gene expression:
- Measure transcription rates without the influence of RNA half-life.
- Ideal for measuring changes in gene expression at early time points (minutes).
- Detect alternate start sites and unannotated genes.
- Identify genes that are poised for transcriptional activation.
- Perform in parallel with transcription factor-targeted ChIP to correlate TF binding with changes in transcription.
