PrimeScript™ 1st strand cDNA Synthesis Kit

cDNA synthesis kit: PrimeScript First Strand cDNA Synthesis Kit

The PrimeScript First Strand cDNA Synthesis Kit contains all of the reagents necessary for synthesis of first-strand cDNA from total or poly(A)+ RNA using PrimeScript Reverse Transcriptase. Choose this cDNA synthesis kit when you want to synthesize full-length cDNA with provided primers (select from oligo dT or random 6-mer primers, both included in the kit.) This cDNA synthesis kit is powered by PrimeScript Reverse Transcriptase, which has exceptionally strong strand displacement activity and efficiently synthesizes cDNA. PrimeScript RTase is robust, versatile and well-suited for applications requiring full-length cDNA such as cDNA library construction. This PrimeScript-based cDNA synthesis kit also works well with other cDNA-dependent techniques including RT-PCR, cDNA probe preparation and real-time quantitative RT-PCR (qRT-PCR or RT-qPCR). The PrimeScript First Strand cDNA Synthesis Kit is ideal for reverse transcription of many different RNA templates, including GC-rich templates and RNAs with high levels of secondary structure.

First introduced in Japan, PrimeScript is now available to scientists worldwide. Over 3,700 peer-reviewed articles have been published in which PrimeScript Reverse Transcriptase was referenced in a variety of scientific applications such as gene expression, gene discovery, transcriptome analysis, miRNA expression and regulation, molecular evolution, virology and microbiology. PrimeScript RTase is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to have low levels of RNase H. Because of the excellent extension capability of PrimeScript RTase, cDNA synthesis reactions can be performed at a lower temperature (42°C), decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.

Applications:

• cDNA synthesis kit for RT-PCR
• First strand cDNA synthesis
• cDNA probe preparation
• Synthesis of cDNA libraries with a high proportion of full-length cDNAs

Features:

• This cDNA synthesis kit includes oligo-dT and random 6-mer primers
• Strong strand displacement and extension capability: PrimeScript RTase is capable of synthesizing long and full-length cDNA molecules (up to 12 kb)
• High specificity: synthesize cDNA at 42°C - low background, high yield, and high quality RNA at a temperature that does not promote RNA degradation
• Outstanding accuracy: Lowest error rate among five commercially available reverse transcriptases tested
• Easy to use: Low rates of non-specific annealing, even on incompletely denatured RNA
• Works on challenging templates: Excellent results even with templates that are GC-rich or have high levels of secondary structure
• Highly sensitive: use less of your precious RNA samples
• Demonstrated success with RT reaction times of 30 min. and 60 min (for longer transcripts).