Probe Based One Step RT-qPCR with UNG & MegaMix Platinum
A comprehensive kit for efficient multiplex RT-qPCR in a single reaction tube. Ideal for multiplex (4-5) assays using dual-labelled hydrolysis probes, works with standard and fast cycling.
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Containing all the components needed to perform multiplex RT-qPCR swiftly and reliably in one reaction.
The kit consists of 2X MMP One-Step RT-qPCR mastermix, containing chemically modified Hot Start Taq DNA polymerase, dUTP and UNG in enhancing buffer; 20X RTase/RI enzyme mix, optimised for amplifying low copy RNA targets; and Just Water, our molecular biology grade water. The presence of thermolabile UNG and dUTP eliminates carryover contamination at room temperature. Unlike other UNG enzymes it is totally and irreversibly inactivated during thermocycling allowing post PCR analysis. This mix has been optimised for multiplex assays utilising dual labelled hydrolysis probes and is compatible with both standard and fast cycling conditions.
Key Features
- Multiplexing—excellent for multiplexed reactions consisting of 4 or 5 targets without compromising performance.
- Eliminate carry over contamination—incorporation of thermolabile UNG and dUTP prevent amplicon contamination from previous runs.
- Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
Applications
- Multiplexed RT-qPCR probe based assays including TaqMan molecular beacons, ScorpionsTM probes.
- Quantification of any RNA template (mRNA, total RNA, viral RNA), low copy number genes.
