Proteome Profiler Human Cytokine Array Kit
A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk.
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This sensitive tool for simultaneously detecting multiple cytokines in once.
Understanding of cellular responses
The Bio-Techne Proteome Profiler Human Cytokine Array Kit boasts an impressive array of features tailored to meet the demands of cytokine profiling. Researchers can simultaneously detect multiple cytokines in a single sample and obtain a holistic view of cellular responses. The kit's high sensitivity ensures the detection of even low-abundance cytokines, facilitating a deeper understanding of biological phenomena. Furthermore, its compatibility with various sample types, including serum, plasma, and cell culture supernatants, enhances its versatility and applicability across diverse research domains.
Review Date: 13 May 2024 | Bio-Techne
Key Benefits
- Detects 36 human cytokines, chemokines, and acute phase proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required. Refer to protocol.
Principle of the Assay
The Proteome Profiler Human Cytokine Array Kit is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
Kit Contents
- Rectangular 4-Well Multi-dish
- Human Cytokine Array, Panel A nitrocellulose membranes spotted with 36 different antibodies to human cytokines, chemokines, and acute phase proteins
- Array Buffer 4
- Array Buffer 5
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Human Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
