ProteoPlex™ 16-well Human Cytokine Array Kit

The ProteoPlex™ 16-Well Human Cytokine Array Kit is designed for multiplex detection and measurement of 12 important human cytokines in parallel from up to 15 experimental samples.
The combination of advanced surface chemistry, well-characterized capture agents, and a highly-sensitive fluorescence-based detection system provides a robust method for comparing relative cytokine abundance or cytokine quantification in serum samples or cell supernatants.

The 12 analytes measured in the kit are pro- and anti- inflammatory cytokines important for the study of immune system regulation and diseases such as asthma and arthritis. Each well on the slide contains a microarray of spotted antibodies with four "spots" for each of the 12 cytokines plus additional spots for positive and negative controls. The replicate spots enable reliable quantitative data from a single sample. A typical experimental design is to process 10 samples and a six-point standard curve.

The simple protocol is familiar to researchers as it is similar to the protocols used for decades in single-analyte immunoassay systems. The 16-well slide format and SensiLight™ fluorescent detection system are compatible with a broad range of microarray scanners.

The ProteoPlex Human 16-Well Cytokine Array Kit is a complete system that includes sample diluent, buffers, cytokine standards, detection antibodies, and SensiLight fluorescent detection reagents. Slide Scanning and Analysis Services are also available. The results are returned within a few business days of receiving slides for analysis.