PureLink™ RNA Mini Kit
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides: • Rapid, non-hazardous RNA purification capabil…
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RNA extraction
The PureLink RNA Extraction kit is very easy to use and works really well, giving awesome results!
Review Date: 3 Dec 2020 | Thermo Fisher Scientific
Extracting RNA
The kit is really easy to use and gives great, reproducible results. I would recommend it to others.
Review Date: 11 Jul 2019 | Thermo Fisher Scientific
RNA purification
I had used this kit continuously for RNA extraction for qPCR quantification. The yield, quality and reproducibility are pretty good. It offers on-column DNase treatment.
Review Date: 26 Jan 2018 | Thermo Fisher Scientific
isolate RNA from cell cultures
Rapid extraction, easy to follow protocol, good yield
Review Date: 15 Nov 2017 | Thermo Fisher Scientific
The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides:
• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications
Rapid and convenient column purification
The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 µg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.
Simplified, nontoxic RNA purification
The PureLink® RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.
The PureLink RNA Mini Kit is recommended to be used together with the Homogenizer (Cat. No. 12183026) designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.
For plant tissues rich in polyphenolics or starch (e.g., pine needles, potato tubers), we recommend using PureLink™ Plant RNA Reagent (Cat. No. 12322012) together with the PureLink RNA Mini Kit.