QIAGEN Plasmid Plus Midi Kit (100)
For the fastest, most convenient purification of up to 10 mg transfection-grade plasmid DNA
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Plasmids extraction
Really easy to use. All of the procedures can be done in one and a half hours. The quality of the plasmids are pretty good, even 260/280 ratio is always around 1.9, but that would not affect transfection efficiency too much.
Review Date: 5 Nov 2018 | QIAGEN
Endotoxin-Free Plasmid DNA Preparation
1. The column is very easy to use. 2. It can obtain high quality plasmid DNA, easy to use for transfection. 3 It can process 20-30 samples same-time, at high efficiency. 4. Only negative point is the results are inconsistent. I called tech support, they said can't use more than 20 ml of culture, But in the past, I got very high yield even from 100 ml of cultured bacteria and sometimes failed to get DNA from 20 ml of culture.
Review Date: 20 May 2014 | QIAGEN
The QIAGEN Plasmid Plus Kits enable ultrafast, large-scale purification of up to
10 mg of highly pure plasmid DNA. The use of a vacuum manifold allows purification of up to 24 samples in parallel, reducing hands-on time. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. The QIAGEN Plasmid Plus Kits also feature a dedicated wash buffer for endotoxin reduction. The plasmid DNA obtained is highly suitable for a multitude of applications, including transfection into sensitive cell lines.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.
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