QIAseq Targeted RNA Panels
Kit containing wet bench-verified assays and reagents for first strand synthesis, molecular barcoding, gene-specific amplification and library preparation for targeted RNA sequencing
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Immunology
I was searching for a kit to facilitate my TCR sequencing from which the TCRseq will be used for my TCRavatar experiments for studying T1D autoimmunity. We tested different types of products from different companies or different homemade methods with few successes due to low detection sensitivity. The kit from QIASEQ surprised me as it allows to detect a low amount of TCR from a low frequency of TCR of interest. The procedure to generate NGS library is fast and least time-consuming, and the data, personally, I feel is the best one I have used.
Review Date: 4 Apr 2023 | QIAGEN
QIAseq Targeted RNA Panels have been developed as a Sample to Insight solution for quantitative gene expression profiling using RNAseq. These panels integrate molecular barcode technology and a two-stage PCR-based library preparation to deliver unbiased and accurate quantification for your digital RNA sequencing results. QIAseq Targeted Panels are wet-bench verified to ensure the highest quality of results.
Features:
- Molecular barcodes removes PCR and library construction bias
- Start with only 25 ng of total RNA
- Simple 1 day library construction workflow
- Use with any Illumina or Thermo-Fisher NGS instrument