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Quantifiler™ Trio DNA Quantification Kit

The Quantifiler® Trio DNA Quantification Kit is a robust kit that enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. This guides selection of the optimal short tandem repeat (STR) analysis chemistry (autosomal, Y-STR, or miniSTR) and streamlines the workflow while increasing downstream analysis…

Thermo Fisher Scientific

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Average Rating 4.7

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Average Rating 4.7

Application Area:

Forensic genetics

DNA quantification kit that revolutionized the forensic world. With a specific degradation grade probe, you can choose the right amount of DNA for PCR.

Review Date: 9 Apr 2018 | Thermo Fisher Scientific

The Quantifiler® Trio DNA Quantification Kit is a robust kit that enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. This guides selection of the optimal short tandem repeat (STR) analysis chemistry (autosomal, Y-STR, or miniSTR) and streamlines the workflow while increasing downstream analysis success rates. The kit includes enhanced quantity and quality indicators that help deliver accurate, sensitive, and specific results that work together with the HID Real Time PCR Analysis Software v1.2 to enable informative and integrated workflow decisions. The Quantifiler® Trio kit and the HID v1.2 software are designed for use with the Applied Biosystems® 7500 Real-Time PCR System.

Highly Accurate

  • Determines the ratio of human male to female DNA, even with minute male and excess female DNA (1:4000 or greater)

Highly Sensitive

  • <1 pg/µL limit of detection. Quantifies DNA concentrations from 0.005 ng/uL to >50 ng/uL, enabling the use of a broad range of concentrations, with flexible protocols for higher concentration samples

Highly Specific

  • Detects human and higher primate DNA

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