Quik Change Site-Directed Mutagenesis
The original QuikChange Site-Directed Mutagenesis Kits eliminate the need for subcloning into M13-based bacteriophage vectors and for ss-DNA rescue. This makes site-directed mutagenesis studies simple and reliable since it allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA.
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Great results and cannot live without it.
Mutagensis of DNA sequencing
The reagents in the kits with good quality. High efficiency to get site-directed mutagenesis through PCR on target nucleotide.
Review Date: 23 Dec 2016 | Agilent Technologies
An expensive product but it works well.
Review Date: 2 Feb 2010 | Agilent Technologies
Review Date: 10 Jul 2009 | Agilent Technologies
The original QuikChange Site-Directed Mutagenesis Kits eliminate the need for subcloning into M13-based bacteriophage vectors and for ss-DNA rescue. This makes site-directed mutagenesis studies simple and reliable since it allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. The XL version of the kit is specialized for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates and features components specifically designed for more efficient DNA replication and bacterial transformation. The QuikChange Multi system allows mutagenesis at multiple sites (up to 5) in a single round, using a single oligonucleotide per site.
- >80% mutagenesis efficiency for 1 site; >55% mutagenesis efficiency for 3 mutations simultaneously
- Efficient and accurate linear amplification minimizes unwanted errors
- Free access to Primer Design Program
- QuikChange Lightning Live Webinar: Accelerate your research without sacrificing mutation efficiency, reliability and accuracy.
For Research Use Only. Not for Use in Diagnostic Procedures.
