RAP, rat recombinant, 500 µg
Protein standard for immunoblotting, immunization, immunoassay and receptor-binding studies. Sequence highly homolog with human RAP. Rat his:RAP:c-myc fusion protein. Ligand binding to RAP is Ca2+ dependent and e.g. lipid receptors can be released from RAP by a buffer containing 10 mM EDTA. Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+).
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Protein standard for immunoblotting, immunization, immunoassay and receptor-binding studies. Sequence highly homolog with human RAP.
Rat his:RAP:c-myc fusion protein.
Ligand binding to RAP is Ca2+ dependent and e.g. lipid receptors can be released from RAP by a buffer containing 10 mM EDTA (Bajari et al. 2005). Furthermore, buffers containing phosphate should be avoided (it would form precipitates with Ca2+).