RapiDxFire qPCR 5X Master Mix GF
Formulated with diagnostic kit developers in mind, RapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X concentration without passive reference dye, this flexible master mix allows more room…
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Formulated with diagnostic kit developers in mind, RapiDxFire™ qPCR 5X Master Mix glycerol free (GF) can be combined with gene-specific primers and hydrolysis probes for immediate use in high-throughput laboratory developed test (LDT) workflows or further processed for lyophilisation for use in point of care (POC) devices. Provided at a 5X concentration without passive reference dye, this flexible master mix allows more room for sample and up to 5 target detection at one time. The RapiDxFire qPCR 5X Master Mix, manufactured in an ISO 13485-certified facility, is available in small development sizes to large scale production batches to assist you throughput the diagnostic test development process.
RapiDxFire qPCR 5X Master Mix GF has been optimised for use with all Biosearch Technologies’ probes (Dual-Labelled BHQ, BHQplus, BHQnova and BHQplex CoPrimers), which all contain Biosearch Technologies’ proprietary BHQ quenchers. BHQ dyes have been proven to dramatically reduce low-level background fluorescence due to highly efficient static quenching between reporter and quencher. BHQ dyes are compatible with reporter fluorescent dyes that span the visible spectrum, allowing for broad flexibility in fluorophore selection. RapiDxFire qPCR 5X Master Mix GF can also be used with any probe-based assay. In addition, the Biosearch Technologies RealTimeDesign software is available online to facilitate the design of qPCR assays. Research use only not for use in diagnostic procedures.
High-performing, lyo-compatible master mix for sensitive pathogen detection.
- 5X dye-free formulation offering flexible reaction setups and protocols
- Sensitive detection down to ~10 genomic DNA copies
- Wide dynamic range for multiplexing
- 48-hour reaction benchtop stability ideal for automated workflows
- Glycerol-free, Triton-free, high concentration, and bulk formulations for adaptable test development and lyophilisation options.
- Manufactured in an ISO 13485-certified facility demonstrating batch to batch reproducibility
- Part of the RapiDxFire 1-step RT-qPCR system for effective detection of viral RNA pathogens
