Recombinant Human Androgen Receptor
Recombinant Human Androgen Receptor
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Product Specifications
Format
Lyophilized powder
Formulation
Lyophilized from a 0.2 µm filtered solution of 0.1 M NaHCO3, 0.5 M NaCl, pH 7.5.
Purification
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity
> 95%
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level
Not determined
Purity determined by
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Preservative
None
Recommended Applications
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc.
Source
Gene Symbols
AR
Species
Human
Accession Number
P10275
Expressed Region
Met1-Lys556
Protein Name & Synonyms
Androgen receptor, Dihydrotestosterone receptor, DHTR, HUMARA, KD, NR3C4, SBMA, SMAX1, TFM.
Preparation
Expression System
E.Coli
Tag
His
Conjugation/Tag Notes
N-terminal 6x histidine tag
Molecular Weight (kDa)
The predicted protein size is 62 kDa, but the actual molecular weight is ∼75 kDa
Storage
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
