Rhodamine Phalloidin
Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC). • Selectively stains F-actin • Excitiation/Emission: 540/565 nm • Superior to antibody staining • Optimal for fixed and permeabilized samples • Very widely cited fluorescent phalloidin conjugate Get Superior Results in Actin Staining Studies Phalloidin is a bicyclic peptide belonging to a…
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Great results but very expensive.
Cell cytoskeleton staining
Rhodamine-Phalloidin stains the cytoskeleton perfectly. Following the official protocol takes time but is straight forward to use. The only thing is the price which is in my eyes quite high as you also need very high amounts compared to calcein or propidium iodide.
Review Date: 23 Jul 2021 | Thermo Fisher Scientific
Amazing product, very reproducible results.
Cell staining / In vitro actin experiments
Gives amazing actin staining in fixed cells. Very good also to be used in in vitro actin assays. Fluorescence is very strong.
Review Date: 24 Mar 2017 | Thermo Fisher Scientific
Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC).
• Selectively stains F-actin
• Excitiation/Emission: 540/565 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
• Very widely cited fluorescent phalloidin conjugate
Get Superior Results in Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides "death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has virtually identical binding properties with actin from different species including plants and animals. Phalloidin binds F-actin with high selectivity while TRITC provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database).
Use in Multiple Applications
Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot® nanocrystals, and Alexa Fluor® conjugates including secondary antibodies. For research use only.
