RNA Complete BCT®

RNA Complete BCT is a direct draw blood collection tube that stabilizes draw-time cell-free RNA and exosome counts for up to 7 days when stored at room temperature. It contains a preservative agent that limits the degradation of white and red blood cells, maximizing plasma yield and maintaining sample integrity during storage, shipping and handling of blood samples. The cell-free RNA and extracellular vesicles (EVs) isolated from stored plasma are suitable for many downstream applications including qualitative and quantitative real-time PCR, Droplet Digital PCR, next-generation sequencing and Nanoparticle Tracking Analysis.

RNA Complete BCT is compatible with the following commercially available plasma nucleic acid isolation kits: Qiagen QIAamp^® Circulating Nucleic Acid Kit, Norgen Plasma/Serum Circulating and Exosomal RNA Purification Kit, and Applied Biosystems MagMAX™ Cell-Free Total Nucleic Acid Isolation Kit. It is also compatible with standard low input RNA library prep kits including the New England BioLabs NEBNext^® Ultra™ II Directional RNA Library Prep Kit and the Illumina TruSeq™ RNA Library Prep Kit.

Features

• Stabilizes cell-free RNA concentrations in samples for up to 7 days of room temperature storage
• Compatible with commercially available total plasma nucleic acid isolation kits and standard low input RNA sequencing library prep kits
• Isolated cell-free fRNA is suitable for downstream applications, including ddPCR and next-generation sequencing
• Isolated extracellular vesicles are suitable for Nanoparticle Tracking Analysis or indirect analysis of EV-associated cargo

Benefits

• Limits the degradation of white and red blood cells, providing sample integrity during storage, shipping, and handling of blood samples
• Room temperature storage reduces costs and complications associated with cold chain shipping
• Eliminates the need for immediate plasma preparation
• Reduces hemolysis and increases plasma yield after storage compared to alternative blood collection tubes