RNAlater - ICE Frozen Tissue Transition Solution
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA. Process previously frozen…
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Good for RNA isolation from a certain fraction of frozen tissue.
Treat frozen organ for further dissection, RNA application toward RNA sequencing
It is very easy to use. A plus is that when the reagent penetrated the tissue, it denatures protein and the tissue become pliable while easy to dissect. We applied it to small and large intestine, the mucosa separation was very clean. RNA quality well preserved. Good reagent for archived frozen samples, especially when RNA from a certain fraction of tissue is needed (requires further dissection). Highly recommend.
Review Date: 24 Jan 2020 | Thermo Fisher Scientific
Reliable and high yield for RNA isolation from a certain fraction of samples.
RNA preservation and isolation
Aside from the recommended use by the manufacture, we use it to isolate RNA from a fraction of archived frozen sample. The sample we treated with the RNAlater ICE was pig intestine. After treatment, the proteins in the intestine were denatured and hardened somehow, making it easy to dissect out the desired fraction. The RNA was preserved and the yield was high. Highly recommend this product.
Review Date: 11 Dec 2019 | Thermo Fisher Scientific
RNAlater®-ICE is a novel reagent for transitioning frozen tissue to a state that is easily processed for extraction of high quality RNA. Frozen tissues are simply submerged in RNAlater®-ICE and allowed to thaw overnight at -20°C. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA.
- Process previously frozen tissues like freshly harvested samples
- Thawing tissues in RNAlater®-ICE protects RNA from degradation
- No more tissue pulverizing with mortar and pestle and awkward transfer of powder to tube
- Easily apportion frozen tissue samples for multiple experiments
- Quick Freezing Tissues Preserves RNA
