Seahorse XF Glycolytic Rate Assay Kit
The XF Glycolytic Rate Assay provides real-time measurements of glycolysis, allowing to detect glycolysis modulation in minutes in live cells.
Receive your quote directly from the manufacturer.
We will continue using the equipment during 2022
Mitocondria
Excellent chemicals
Review Date: 13 Dec 2021 | Agilent Technologies
Great results.
Seahorse assay
The product is easy to use.
Review Date: 9 Dec 2021 | Agilent Technologies
Very good.
Analyze proportion of glycolytic vs oxidative metabolism in primary cells
Results are consistent and reproducible. The kit it easy to use with primary cells. It can be used in conjunction with the cell mito stress test (in glucose-free media) to assess access to internal glycogen stores. The customer service used to be better with our previous representative, however, as long as you are using the kit "on label" there is good support.
Review Date: 26 Nov 2019 | Agilent Technologies
The XF Glycolytic Rate Assay provides real-time measurements of glycolysis, allowing to detect glycolysis modulation in minutes in live cells. Researchers use this important assay to advance their understanding of immune cell activation, cancer cell metabolism and cell proliferation. The assay reports multiple key parameters, including basal glycolysis (a physiological rate) and compensatory glycolysis. Compensatory glycolysis is the glycolytic rate that cells can achieve when mitochondrial respiration is inhibited, which is indicative of cellular ability to manage energy demand under stress.
This assay should be performed using XF pH-defined media. Please refer to assay user guide to select the correct media.
Features:
- Each kit contains sufficient materials for performing 6 full plate assays
- It calculates and subtracts mitochondrial-produced acidification for a precise measure of glycolytic rate
- It detects metabolic switching (such as Warburg effect) by reporting relative activity of mitochondrial respiration and glycolysis pathways
- It measures before and after effects of metabolic modulators on glycolytic rates within a single assay well
- This assay is best performed with Seahorse XF DMEM medium, pH 7.4 (Cat No. 103575-100) or Seahorse XF RPMI medium, pH 7.4 (Cat No. 103576-100). For other assay media options, refer to XF buffer factor protocol quick reference guide
- Data analysis tool, the Seahorse XF Glycolytic Rate Assay Report Generator, is provided to simplify and transform raw data into organized, shareable summary reports anyone can understand.
- The assay is for use with an XFe96, XF96, or XFe24 Analyzer. not compatible with XF24 and XF24-3 analyzers. For more information, please see the Agilent Seahorse XF Glycolytic Rate Assay Kit product website
