Seahorse XF Real-Time ATP Rate Assay Kit

The Agilent Seahorse XF ATP Real-Time rate assay is the only assay that measures and quantifies the rate of ATP (adenosine triphosphate) production from glycolysis and mitochondria simultaneously using label-free technology in live cells, in real time. This assays can be used as a starting point to understand metabolism-supported cellular function. It provides an overview of the energetic phenotype of the cells and allows for quantitative comparison of energy engines (mitochondria and glycolysis) in basal state.

The assay employs a sequential injection of Oligomycin and Rotenone/Antimycin A, and reports multiple parameters, including: glycoATP production rate, mitoATP production rate, total ATP production rate and XF ATP rate index.

This assay should be performed using XF pH-defined media. Please refer to assay user guide to select the correct media.

Features:

• Each kit contains sufficient materials for performing 6 full plate assays
• This assay has broad applications. E.g.:
  • Assessing metabolic phenotypic changes and revealing pathway contributions during cell activation, proliferation, and differentiation (e.g., T-cell activation)
  • Evaluate or screen for compound effect on cellular ATP production from mitochondrial respiration and glycolysis simultaneously
• This assay is validated and best performed with Seahorse XF DMEM medium, pH 7.4 (Cat No. 103575-100) or Seahorse XF RPMI medium, pH 7.4 (Cat No. 103576-100). For other assay media options, refer to XF buffer factor protocol quick reference guide
• Kit components are reconstituted directly in assay medium – eliminating the need for DMSO or other solvents
• Kits are rigorously tested and validated for every lot with cells to deliver consistent and robust results – eliminating the cost associated with failed experiments.
• The Seahorse XF Real-time ATP Rate Assay Report Generator is provided to simplify data analysis, and transform raw data into organized, shareable and easy to understand summary reports.