Seahorse XFe24 Analyzers
XFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.
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Promising results
Immunology
Immunometabolism was always neglected before Seahorse XFe Analyzer. I used Seahorse XFe96 Analyzer and applied it in the field of immunotoxicology and showed in my research articles how the study of Immunometabolism of cells in presence of nanoparticles gets affected. In the future, I expect in drug discovery and toxicology studies seahorse XFe Analyzer will be one of the key instruments. Publications Published 1) Dey, A.K., et al., Impact of Gold Nanoparticles on the Functions of Macrophages and Dendritic Cells. Cells, 2021. 10(1). 2) Dey, A.K., et al., Tuning the Immunostimulation Properties of Cationic Lipid Nanocarriers for Nucleic Acid Delivery. Frontiers in Immunology 12.
Review Date: 12 Feb 2023 | Agilent Technologies
Great post-purchase care
Analyze metabolic capacity of tumor cell lines
The Agilent team gave an incredible introduction to the system and how to perform experiments following the purchase of our Ailent Seahorse xfe96, in addition to describing other applications that we had not yet considered. The consumable purchase plan is convenient as well.
Review Date: 9 Dec 2021 | Agilent Technologies
Overall, it is a very good equipment for analyzing mitochondrial metabolism!
Mitochondrial respiration
It is easy to use. We got a lot of good data using the equipment, a few high-quality papers have been published.
Review Date: 17 Nov 2021 | Agilent Technologies
Reliable, sensitive tool for cellular metabolism research!
Analyze glycolysis and mitochondrial respiration rates in cell monolayers
The Seahorse XFe96 analyzer was a valuable tool for my research on BMSC cellular metabolism with aging. With the help of user guides and technical support, my mentor and I were able to optimize a protocol and generate useful data. Additionally, the 96-well capacity of this instrument and associated consumables allowed for large numbers of technical replicates with multiple experimental groups on the same plate. The machine and software were easy to use for protocol setup and group labeling, allowing for good record-keeping and data mining during the analysis steps. This is one of the best tools to have in cellular biology and metabolic research!
Review Date: 24 Sept 2021 | Agilent Technologies
Worth the price.
All aspects of cell analysis
Excellent.
Review Date: 19 Jun 2021 | Agilent Technologies
Great technology! It changed my life
Cell biology
It is a game-changer in your metabolism assays, it helps you to better understand how mitochondria are working in your cells or tissues.
Review Date: 9 Feb 2021 | Agilent Technologies
Great way to see the effect on cell faith and recovery after treatments.
Mitochondrial research
Cellular metabolism is a critical factor in stem cell research with recent reports suggesting that both metabolic status and cell culture conditions can significantly alter the differentiation trajectory of stem cells, as well the effect of drugs like chemotherapy and the recovery of cells with stem cells treatment.
Review Date: 9 Feb 2021 | Agilent Technologies
Must for cancer metabolism research.
Metabolism
Must for metabolism experiment with clarity in real time.
Review Date: 2 Feb 2021 | Agilent Technologies
Great and precision results. A key to success for drug discovery.
Cell metabolism studies
There are different models of the Agilent Seahorse available on the market, among them I found the best one is the Agilent Seahorse XFe96 Analyzer because the user can use 96 well plate means to measure the 96 samples in one run. We use the Agilent Seahorse XFe96 to measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. The oxygen consumption rate (OCR) and extracellular acidification rates (ECAR) are key indicators of mitochondrial respiration and glycolysis as well as the ATP production rate. This measurement together provides a systems-level view of cellular metabolic function in cultured cells and in vitro samples. The story is not over here, one more powerful software tool is also available in the market like MITOsym from simulation plus. MITOsym uses for the mathematical modeling of the data obtained from the Seahorse XFe analyzer for cellular respiration and bioenergetics. The results obtained from the Seahorse XFe analyzer is very reliable and useful for the development and discovery of new drugs for cancer.
Review Date: 14 Nov 2020 | Agilent Technologies
Intuitive instrument for live cell analysis.
Live cell analysis of mitochondrial function
The Seahorse XFe96 cell analyzer is a benchtop instrument that is mainly intended for our group to analyze mitochondrial function in live cells. It is quite intuitive and the user interface is easy to use with simple directions to operate the analyzer. The data generated is medium throughput and will be adequate for a research group. However, given the cost of consumables from Agilent, researchers may be tempted to use certain reagents sold by other manufacturers.
Review Date: 16 Mar 2020 | Agilent Technologies
XFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.
The measurement of cellular bioenergetics on live cells enables time-resolved analysis and testing of multiple conditions per assay well. XF assays provide increased throughput and use less sample compared to conventional respirometry techniques. XFe Analyzers are available in two formats- 96-well for maximum throughput and flexibility, and 24-well for specialty samples such as islets and small model organisms.
Features:
- Live-Cell, Real-Time Assays
- Detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing
- Direct Measure of Energy Metabolism
- Validate results from protein/gene expression experiments with a functional assay
- Label-free platform
- No staining, analyte extraction, or destructive sample processing required
- Minimal Sample Required
- As few as 5000 cells/well (varies by cell type and instrument)
