Sequencing Grade Modified Trypsin
Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in addition…
Proteolytic enzyme for downstream LCMS
Works. Reproducible results. DDA or DIA, this enzyme gets the job done.
Review Date: 17 Nov 2021 | Promega Corp.
Proteome in mitosis
Very efficient enzyme, missed cleavages happen occasionally.
Review Date: 19 May 2021 | Promega Corp.
Proteomics
Have use within our service facility for over 10 years and we were always happy with the product and the reproducibility of the data. The aftercare was great!
Review Date: 27 Jun 2019 | Promega Corp.
Protease for proteomics applications
The sequencing grade modified trypsin comes in handy glass vials with 5 x 20 micrograms, either lyophilized or frozen. Excellent activity and high batch-to-batch reproducibility. Great both for complex protein mixtures and for individual proteins.
Review Date: 31 Oct 2017 | Promega Corp.
proteomics
It is a nice protease which is resistant to auto-proteolysis and gives digestion to your protein.
Review Date: 5 Apr 2017 | Promega Corp.
Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.
The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in additional peptide fragments that could interfere with database analysis of the mass of fragments detected by mass spectrometry.
Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.
Sequencing Grade Modified Trypsin Features & Benefits:
- TPCK Treatment Followed by Affinity Purification - Elimination of chymotrypsin activity enables distinct and consistent data.
- Stability - Ensured up to five freeze-thaw cycles.
- Reliable and Customer-Proven - Referenced in thousands of papers.
- Alternative Formats - Flexibility depending on experimental design and scope.