Products & ReviewLife Sciences

Sequencing Grade Modified Trypsin

Promega Corp.V5111, V5117, V5113Available: Worldwide

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.   The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in addition…

Request Pricing
Promega Corp.

Receive your quote directly from the manufacturer.

Average Rating 4.5

|5Scientists have reviewed this product

Ease of Use
After Sales Service
Value for Money
Write your own review

No regrets. Buy now!

 

Average Rating 5.0

Application Area:

Proteolytic enzyme for downstream LCMS

Works. Reproducible results. DDA or DIA, this enzyme gets the job done.

Review Date: 17 Nov 2021 | Promega Corp.

My key tool.

 

Average Rating 5.0

Application Area:

Proteome in mitosis

Very efficient enzyme, missed cleavages happen occasionally.

Review Date: 19 May 2021 | Promega Corp.

Would recommend!

 

Average Rating 4.7

Application Area:

Proteomics

Have use within our service facility for over 10 years and we were always happy with the product and the reproducibility of the data. The aftercare was great!

Review Date: 27 Jun 2019 | Promega Corp.

Enzyme to be trusted

 

Average Rating 4.7

Application Area:

Protease for proteomics applications

The sequencing grade modified trypsin comes in handy glass vials with 5 x 20 micrograms, either lyophilized or frozen. Excellent activity and high batch-to-batch reproducibility. Great both for complex protein mixtures and for individual proteins.

Review Date: 31 Oct 2017 | Promega Corp.

Nice and reliable product

 

Average Rating 3.3

Application Area:

proteomics

It is a nice protease which is resistant to auto-proteolysis and gives digestion to your protein.

Review Date: 5 Apr 2017 | Promega Corp.

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues.

The stringent specificity of trypsin is essential for protein identification. Native trypsin is subject to autolysis, generating pseudotrypsin, which exhibits a broadened specificity including a chymotrypsin-like activity. Such autolysis products, present in a trypsin preparation, would result in additional peptide fragments that could interfere with database analysis of the mass of fragments detected by mass spectrometry.

Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.


Sequencing Grade Modified Trypsin Features & Benefits:

  • TPCK Treatment Followed by Affinity Purification - Elimination of chymotrypsin activity enables distinct and consistent data.
  • Stability - Ensured up to five freeze-thaw cycles.
  • Reliable and Customer-Proven - Referenced in thousands of papers.
  • Alternative Formats - Flexibility depending on experimental design and scope.

Product Overview

Links