SialEXO™
SialEXO is a sialidase mix for complete removal of sialic acids from native glycoproteins. SialEXO is supplied as a lyophilized powder with no preservatives added. It is available in 2000 unit vials for release of sialic acids on 2 mg glycoprotein.
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Reliable sialidase, also useful for complex jobs
Sialidase mix for the removal of sialic acids from glycoproteins
SialEXO is a sialidase mix, which can be used for the complete removal of sialic acids from native glycoproteins. Working perfectly fine in a range of buffers, the enzyme has been extensively used in the lab. Great reproducibility even on very complex glycoproteins. Fast and reliable. Can be removed from the final mixture as the enzyme is His-tagged.
Review Date: 27 Apr 2018 | Genovis
SialEXO is a combination of two sialidases for efficient desialylation of O- and N-glycosylated proteins. The mix is composed of sialidases for high efficiency in hydrolyzing α2-3, α2-6 or α2-8 bonds. SialEXO hydrolyzes glycoproteins under native conditions and displays activity in a pH range from 6.5 to 9. Enzymes in SialEXO are derived from Akkermansia muciniphila and expressed in E. coli. The enzymes in the mix contain a His-tag and the molecular weights of the components are 43 kDa and 66 kDa, respectively.
Unit Definition
One unit of SialEXO hydrolyzes sialic acids from ≥ 90% of 1 µg glycoprotein (fetuin) when incubated in 20 mM Tris pH 6.8 at 37 °C for 2 h.
Content and Storage
SialEXO is supplied lyophilized in TBS pH 7.6, with no preservatives added.
SialEXO is shipped cold and should be stored at –20 °C upon arrival.
After reconstitution SialEXO is stable for 1 month at +4-8 °C.
