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SingleShot™ Cell Lysis Kit, 500 x 50 µl rxns

Bio-Rad1725081

SingleShot™ Cell Lysis Kit, 500 x 50 µl reactions

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Use the SingleShot Cell Lysis Kit to rapidly generate cell lysates that are suitable for RT-qPCR analysis without RNA purification.

Kit works on 10 to 100,000 cells in 20 minutes while providing the following key benefits:

  • Cell lysate from 10 to 100,000 cells ready in 20 minutes
  • Complete removal of genomic DNA while preserving RNA integrity
  • Optimal accuracy and linear dynamic range of qPCR data across genes
  • Results comparable to using purified RNA, including dynamic range
  • Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr )
  • SingleShot RNA Control included to ensure optimal cell and lysate inputs

Optimized Sample Input

The SingleShot Cell Lysis Kit maximizes cell lysate and cDNA inputs in RT-qPCR reactions:


Kit Contents

Refer to the user manual for details on reaction setup and troubleshooting tips.

Cell Lines Validated for Use with the SingleShot™ Cell Lysis Kit

The SingleShot™ Cell Lysis Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

RT-qPCR Step Reagents (Sold Separately)

Reverse Transcription

  • iScript™ Advanced cDNA Synthesis Kit for RT-qPCR ( 172-5038 )

Two-Step RT-qPCR Reagents

  • SsoAdvanced™ Universal SYBR® Green Supermix ( 172-5271 )
  • SsoAdvanced™ Universal Probes Supermix ( 172-5281 )

One-Step RT-qPCR Reagents

  • iTaq™ Universal SYBR® Green One-Step Kit ( 172-5151 )

  • iTaq™ Universal Probes One-Step Kit ( 172-5141)


  • PrimePCR™ Reverse Transcription Control Assay

Related Products

The SingleShot Cell Lysis Kits belong to the family of SingleShot™ Cell Lysis RT-qPCR Kits :

  • SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit ( 172-5095 ) and SingleShot Probes One-Step Kit ( 172-5070 )
  • SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit ( 172-5085 ) and SingleShot Probes Kit ( 172-5090 )
  • SingleShot Cell Lysis Kit — available for 100 reactions ( 172-5080 )

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