SingleShot™ Cell Lysis Kit, 500 x 50 µl rxns
SingleShot™ Cell Lysis Kit, 500 x 50 µl reactions
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Use the SingleShot Cell Lysis Kit to rapidly generate cell lysates that are suitable for RT-qPCR analysis without RNA purification.
Kit works on 10 to 100,000 cells in 20 minutes while providing the following key benefits:
- Cell lysate from 10 to 100,000 cells ready in 20 minutes
- Complete removal of genomic DNA while preserving RNA integrity
- Optimal accuracy and linear dynamic range of qPCR data across genes
- Results comparable to using purified RNA, including dynamic range
- Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr )
- SingleShot RNA Control included to ensure optimal cell and lysate inputs
Optimized Sample Input
The SingleShot Cell Lysis Kit maximizes cell lysate and cDNA inputs in RT-qPCR reactions:
Kit Contents
Refer to the user manual for details on reaction setup and troubleshooting tips.
Cell Lines Validated for Use with the SingleShot™ Cell Lysis Kit
The SingleShot™ Cell Lysis Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:
RT-qPCR Step Reagents (Sold Separately)
Reverse Transcription
- iScript™ Advanced cDNA Synthesis Kit for RT-qPCR ( 172-5038 )
Two-Step RT-qPCR Reagents
- SsoAdvanced™ Universal SYBR® Green Supermix ( 172-5271 )
- SsoAdvanced™ Universal Probes Supermix ( 172-5281 )
One-Step RT-qPCR Reagents
- iTaq™ Universal SYBR® Green One-Step Kit ( 172-5151 )
- iTaq™ Universal Probes One-Step Kit ( 172-5141)
- PrimePCR™ Reverse Transcription Control Assay
Related Products
The SingleShot Cell Lysis Kits belong to the family of SingleShot™ Cell Lysis RT-qPCR Kits :
- SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR® Green One-Step Kit ( 172-5095 ) and SingleShot Probes One-Step Kit ( 172-5070 )
- SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR® Green Kit ( 172-5085 ) and SingleShot Probes Kit ( 172-5090 )
- SingleShot Cell Lysis Kit — available for 100 reactions ( 172-5080 )
