Sirius 2 LB 9526 Tube Luminometer
THE FLEXIBLE SOLUTION
High performance luminescence detection
Sirius 2 LB 9526 is a compact and highly sensitive tube luminometer. Light collection is maximized through both the close proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. The luminometer is upgradeable with up to two injectors, making it perfect for flash assays, and can be equipped with an intuitive touch screen computer, enabling stand-alone operation. The Sirius 2 supports all basic luminescence technologies including flash-type Luminescence, glow-type Luminescence, bioluminescence and chemiluminescence. The Sirius 2 detects less than 0.5 zmol of firefly luciferase (1 amol ATP/tube). Light collection is maximized through the close proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. The detector is operated in photon counting mode, which ensures a very low background signal for excellent signal to noise ratio and the highest linearity. The sample chamber is designed for performance and flexibility: from 35 mm petri dishes to microfuge format to 20 mL vials, just pick the sample format that fits your application best. The optional sample holders enable accurate injection into both, microcentrifuge tubes and standard luminescence tubes. The system is compatible with a variety of sample formats: Standard 12 mm luminescence tubes (55 and 75 mm) Microcentrifuge tubes (1.5 and 2 mL) Scintillation vials (up to 20 mL) 35 mm Petri dishes The touchscreen-driven software is very intuitive and operated by touch gestures. The easy-to-use software provides advanced features, such as handling of replicates, dual assay calculations, and display of graphics. Data can be downloaded easily onto a USB stick or printed. The results can be exported for further calculation in Excel or other programs. Closing of the sample drawer automatically initiates the measurement. There´s no need to press any key. The automatic light shutter protects the detector from ambient light. This makes the system ready for measurement from the moment the door is closed. This means that there is no need to set a long delay before the measurement. This is necessary with most other tube luminometers so that the background can return to the initial level after the door has been opened. The system is available with up to two syringe-type automatic reagent injectors. The injection volume range is 20–500 μL. This very broad volume range allows the Sirius 2 to adapt to any assay using injectors. Ready-to go sample holders for precise injection are available for 1.5 ml and 2 ml microfuge tubes, as well as for 55 mm and 75 mm, 12 mm diameter tubes (standard format for most luminescence assays).
Review Date: 26 Jul 2022 | BERTHOLD TECHNOLOGIES GmbH & Co. KG
Measure ATP in marine microbes
I was amazed at the help received from the manufacturer (Berthold Technologies) when I reactivated a Berthold Sirius Luminometer. I find that the sensitivity of the instrument is comparable to any new instrument.
Review Date: 26 May 2020 | BERTHOLD TECHNOLOGIES GmbH & Co. KG
Sirius 2 LB 9526 is a compact and highly sensitive tube luminometer. Light collection is maximized through both the close proximity of the sample to the detector and the use of optimized light reflectors surrounding the sample vial. The luminometer is upgradeable with up to two injectors, making it perfect for flash assays, and can be equipped with an intuitive touch screen computer, enabling stand-alone operation.
The system is the perfect choice for all luminescent flash and glow applications performed in single sample format, for example reporter gene assays, dual-reporter assays, ATP assays, cell proliferation, cytotoxicity and biomass assays, or environmental toxicity and mutagenicity assays.
Sirius 2 benefits at a glance:
- Results you can trust: The Sirius 2 is equipped with a high-sensitivity detector that can detect less than 0.5 zmol firefly luciferase.
- Maximise your benchtop space: PC-free operation (Sirius 2 Touch) and ultra-compact 15 cm wide footprint that fits any laboratory setting.
- Quick-start functions: Closing the sample drawer automatically initiates the measurement with no need to press any key. An automated light shutter protects the detector from ambient light, making the instrument ready to measure from the moment the door is closed.
- Advanced touchscreen operation: The intuitive touch-screen interface of the Sirius 2 Touch allows easy editing of experimental conditions, handling of replicates, dual assay calculations, and display of graphics.
Applications:
- Reporter gene assays
- Luminescent immunoassays
- ATP quantification
- Caspase assays
- Kinase assays
- Toxicity and mutagenicity of water samples