Skeletal Muscle Differentiation Kit

The world’s first protocol to differentiate human pluripotent stem cells to skeletal muscle with high yields and without cell sorting or genetic manipulation. Myotubes are contractile, express typical muscle markers and show striated sarcomeres. The process yields almost pure populations of myogenic cells; it is highly efficient and works robustly with all pluripotent stem cell lines – critical prerequisites for effective cell-based disease modeling and drug development.

Benefits

• Achieves typically 70% MF20-positive myotubes

• Simple 3-step process of media change and cell passaging

• No cell sorting steps required

• No transfection of myogenic transcription factors

• Tested on a wide range of embryonic & induced pluripotent stem cell lines