SPRIselect Reagent Kit for DNA Size Selection
SPRIselect is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection for fragment library preparation for Next Generation sequencing.
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Great, but hard to get!
DNA fragmentation
I used the SPRI beads to generate fragmented DNA for a study that we were doing and I enjoyed the performance of these beads. Also, I like that these can be stored at room temperature unlike some of the other brands that I have which have to be kept at 4C. The results so far are really consistent operator to operator. However, at the time of purchase, it did take more than a couple weeks for them to actually arrive which was not ideal.
Review Date: 18 Jun 2021 | Beckman Coulter Life Sciences
SPRIselect is a SPRI-based chemistry that speeds and simplifies nucleic acid size selection for fragment library preparation for Next Generation sequencing. In this process, size selection is required to produce a uniform distribution of fragments around an average size. Using SPRIselect, the size distribution can be adjusted to suit the application and platform used. The process can be scaled for low to high throughput workflows. Used manually or automated on a liquid handling system such as the Biomek Laboratory Automation Workstation, SPRIselect will provide rapid and consistent size selection suitable for most applications.
Features:
- Tunable from 150 to 800 base pairs
- Efficient mag bead technology eliminates the need for gels, chips or special instrumentation
- Easily adjust for specific application and sequencer
- Consistent results, reproducible sizing between runs and reagent lots
- Scalable from manual to automated for highthroughput processing in 96-well plates
- Proven proprietary technology is already the gold standard for purification steps in Next Gen Sequencing (NGS) sample prep
