StrataClone Blunt PCR Cloning Kit
The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. These kits are available for both blunt-end and UA cloning.
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To Clone High Fidelity PCR products into a vector
I love this kit! Especially when I am doing CRISPR, that means I need to subclone the high fidelity amplified genomic locus sequence, to check the intended mutations, this is the only kit I use! Way better than other company's products, works every time!
Review Date: 15 Dec 2014 | Agilent Technologies
The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. These kits are available for both blunt-end and UA cloning. Additionally, we have combined the highest accuracy in PCR amplification with easy, reliable, and affordably priced topoisomerase-based cloning in our StrataClone Ultra PCR Cloning System, which includes the PfuUltra II Fusion Hotstart DNA Polymerase.
- High efficiency yields >95% insert positive clones
- Clone both long and short amplicons (500 bp – 9.2 Kb) with the same kit
- With Kanamycin and Ampicillin Resistance Markers
For Research Use Only. Not for Use in Diagnostic Procedures.
