Sulfo-NHS-LC-Biotin 100 mg
Sulfo-NHS-LC-Biotin, Sulfosuccinimidyl 6-(biotinamido) Hexanoate, is a long chain, water soluble biotinylation reagent that is reactive toward primary amine groups. Similar to Sulfo-NHS-Biotin, Sulfo-NHS-LC-Biotin is designed to target cell surface proteins. The sodium sulfonate groups lend water solubility to Sulfo-NHS-LC-Biotin and prevent it from crossing the lipid bi-layer of the cell membrane; whereas NHS-LC-Biotin can bi…
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Reactive biotin ester for a reasonable price
Cell-surface labelling of proteins for proteomic applications
Sulfo-NHS-LC-Biotin can be used for the labelling of cell surface proteins of cultured cells and for the labelling of single proteins. The reagent provided by ProteoChem is highly reactive and easy to use. The sulfo group prevents the reagent from crossing cell membranes, making it a great tool for the study of cell surface proteins.
Review Date: 10 Nov 2017 | Proteochem
Sulfo-NHS-LC-Biotin, Sulfosuccinimidyl 6-(biotinamido) Hexanoate, is a long chain, water soluble biotinylation reagent that is reactive toward primary amine groups. Similar to Sulfo-NHS-Biotin, Sulfo-NHS-LC-Biotin is designed to target cell surface proteins. The sodium sulfonate groups lend water solubility to Sulfo-NHS-LC-Biotin and prevent it from crossing the lipid bi-layer of the cell membrane; whereas NHS-LC-Biotin can biotinylate intracellular proteins. The resulting biotinylated protein can then be detected when used in conjunction with streptavidin and Immobilized Streptavidin.
The "long chain" Sulfo-NHS-LC-Biotin and Sulfo-NHS-Biotin both have the same chemical functionality. However, Sulfo-NHS-LC-Biotin will provide a longer spacer arm that is designed to overcome any steric hindrances that may be present between the labeled protein(s) and streptavidin (ProteoChem product # e7105-5mg). Often, the best results are determined by empirically testing each reagent for your specific application.