SunCollect System
Sample Preparation for MALDI-MS
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Workhorse for MALDI target spotting
Versatile sample preparation device
The SunCollect System from SunChrome is used for spotting of UHPLC-fractions on MALDI target plates. The versatile systems allows for a dense spotting scheme (>3000 spots per plate) with high reproducibility. Easy to use software. The droplet deposition by quickly touching the plate surface yields very good results. Importantly, the same device can also be used as fraction collector and as MALDI sprayer for imaging applications.
Review Date: 12 Dec 2017 | SunChrom
Easy to use and reliable instrument
Offline fractionation of complex peptide mixtures and MALDI spotting
The SunCollect System was used to perform offline UPLC-based peptide separation and spotting on MALDI target plates. Great flexibility in spot numbers, density and size. Easy to setup pump for the addition of matrix. Generation of distinct spots, even with very small volumes and when spotting densly (up to 4000 spots per MALDI target plate). The system can also be used for fraction collection and a combination of spotting and fraction collection.
Review Date: 29 Mar 2017 | SunChrom
he SunCollect sample preparation device unifies four discrete instruments in one single unit: SunCollect can be utilized for:
- MALDI-Spotting
- Micro and Nano fraction collection
- Continuous eluate and matrix deposition
- Spraying of matrix and enzyme solutions
SunCollect is a compact unit that requires only a small amount of space and is completely biocompatible, since all samples and collected fractions are in contact only with PEEK and quartz.
The sample preparation is often the limiting factor for a successful MALDI analysis and can be time consuming in some cases. SunCollect is engineered for high precision in combination with a very fast movement. The spotting needle switches from spot to spot in less than a second while depositing very small and precise spots of <10 nl volume at the same time.
When Micro-HPLC or Nano-HPLC is employed to separate samples, the components of the sample are isolated as fractions in the same way as when analytical scale HPLC is used. In many laboratories, the fractions are deposited into microtiter plate (MTP) wells or spotted onto MALDI-MS targets for further investigation. The SunCollect MALDI-Spotter offers outstanding performance for collecting fractions using either approach. If desired, a single fraction can be saved in both formats at the same time by a unique “dual needle-splitter” design. When this approach is used, most of the fraction can be collected in the MTP well and a small volume can be spotted on the MALDI target including the matrix solution. Even if the volume of the fraction is large, the entire fraction can be collected and no eluant is lost. The control software is designed to be user-optimised and extremely simple to operate. It is very easy to enter new sample collection protocols or create new target formats. Moreover, a large variety of MALDI targets and MTPs of all common manufacturers are available preassigned.
