Tagged Protein Quantification Kit | His-Tag (SKU: AK-HIS-004)
The His-Tag Protein Quantification Kit extends the Amperia™ Protein Quantification System for direct, tag-specific measurement of His-tagged recombinant proteins. Using a premix competition format with GenScript’s THE™ His Tag Monoclonal Antibody, the kit delivers accurate, reproducible results across both crude lysates and purified samples, with up to 80 tests per kit.
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Designed for scientists working in recombinant protein expression, purification, and screening, the His-Tag Protein Quantification Kit provides a ready-to-use solution for rapid, reliable quantification of His-tagged proteins.
Each kit includes pre-coated sensor strips, assay plates, and reagents for seamless integration with the Amperia™ system. The assay follows a premix competition format in which His-tagged proteins compete with HRP-labelled His-tagged detection reagents for anti-His capture sites on the sensor. The resulting inverse electrochemical signal allows quantification across a broad dynamic range, even in crude lysates.
Key Features:
- Ready-to-use format tailored for the Amperia™ system
- Tag-specific quantification using GenScript THE™ His Tag Monoclonal Antibody
- Premix competition format with inverse-signal detection
- Up to 80 tests per kit (5 sensor strips, 10 plates included)
- Compatible with crude lysates and purified samples
- Provides consistent, repeatable measurements.
Applications:
- Protein expression and purification monitoring
- Screening of His-tagged constructs or clones
- Optimisation of production conditions
- Comparative analysis of His-tagged variants
- Process development and yield assessment
