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The Interleukin 33 (Human) ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-33.
Method
An anti-human IL-33 coating antibody is adsorbed onto microwells. Human IL-33 present in the sample or standard binds to antibodies adsorbed to the microwells. Following incubation unbound biological components are removed during a wash step. A biotin-conjugated anti-human IL-33 antibody is added and binds to human IL-33 captured by the first antibody. Following incubation unbound biotin-conjugated anti-human IL-33 antibody is removed during a wash step. Streptavidin-HRP is added and binds to the biotin-conjugated anti-human IL-33 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human IL-33 present in the sample or standard. The reaction is terminated by addition of acid and absorbencies measured at 450 nm. A standard curve is prepared from 7 human IL-33 standard dilutions and human IL-33 sample concentration determined.
