Thermo Scientific™ NE-PER™ Nuclear and Cytoplasmic Extraction Reagents
The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours.
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The NE-PER Kit is a nuclear protein extraction method that involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilize and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The kit typically yields 200 to 500 µg cytoplasmic protein and 100 to 200 µg nuclear protein (at a concentration of 1 mg/mL).Once desalted or diluted, the isolated proteins can be used to perform immunoassays and protein interaction experiments, such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.
Features:
- Fast—obtain nuclear and cytoplasmic fractions of soluble proteins in less than two hours
- Proven—the NE-PER Reagent Kit is referenced in more than 950 distinct publications
- Versatile – nuclear protein from either cultured cells or tissues (intended for fresh samples only)
- Convenient—simple instructions do not require ultracentrifugation over gradients
- Compatible—use for downstream assays, including Western blotting, gel-shift assays, protein assays, reporter gene assays and enzyme activity assays
