Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP, which produces a strong chemiluminescent signal in the presence of peroxide that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blot analysis.
Features:
- Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
- Higher sensitivity—detect targets down to the low-picogram level
- Longer signal duration—sustained light output for as long as 5 hours
- More imaging options—X-ray, CCD or laser-based imagers
- More affordable—high quality and performance at a lower price than other suppliers' similar substrates
