Products & ReviewSeparations

Thermo Scientific™ ProPac™ HIC Protein and Peptide Column

Thermo Fisher ScientificIQLAALGABXFAITMBBM

Hydrophobic Interaction Chromatography Columns for the Separation of Large BiomoleculesHydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.The ProPac® HIC-10 column is a high-re…

Request Pricing
Thermo Fisher Scientific

Receive your quote directly from the manufacturer.

Ease of Use
After Sales Service
Value for Money
Write your own review

Hydrophobic Interaction Chromatography Columns for the Separation of Large Biomolecules

Hydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.

The ProPac® HIC-10 column is a high-resolution, high-capacity, silica-based HIC column that provides greater hydrolytic stability under the highly aqueous conditions used in HIC. Examples include the separation of bovine serum proteins, snake venom proteins, enzymes, human skeletal muscle protein (HSMP), monoclonal antibodies, pancreatin, and thrombin and peptide applications including tryptic digests of proteins.


ProPac® HIC Protein and Peptide Column Features:

  • Based on 5 µm ultrahigh purity spherical silica gel particles, with 300 Å pores
  • Proteins separated under non-denaturing conditions
  • High protein loading capacity for protein purification applications
  • Proprietary multidentate silica bonding for enhanced hydrolytic stability
  • High-resolution HPLC separation of proteins and peptides
  • Wide range of applications

Column Details:

  • Phase: HIC
  • Particle Size: 5 µm
  • Pore Size: 300 Å
  • Length: 75, 100, 250 mm
  • ID: 2.1, 4.6, 7.8 mm

Product Overview

Links