Thermo Scientific™ T-PER™ Tissue Protein Extraction Reagent
T-PER Tissue Protein Extraction Reagent enables mild extraction of total protein from tissue samples.
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T-PER Tissue Protein Extraction Reagent contains a proprietary mild detergent to maximize protein solubilization from mammalian tissue samples by homogenization, without compromising function. The simple composition is compatible with additives such as protease inhibitors, salts, reducing agents, chelating agents, providing versatility for many different sample types and lysis applications. Cell lysates prepared with T-PER Reagent are directly compatible with reporter assays (e.g., luciferase, beta-galactosidase, chloramphenicol acetyl transferase), protein kinase assays (e.g., PKA, PKC, tyrosine kinase), immunoassays (e.g., Western blots, ELISAs, RIAs) and/or protein purification procedures. T-PER Reagent was validated for protein yield in both fresh and frozen tissue.
Features:
- Simple procedure—just homogenize tissue sample in 1:20 (w/v) of tissue to T-PER Reagent, then centrifuge to pellet cell or tissue debris
- Mild and convenient—detergent is dialyzable for quick and easy removal; can be used with additional components (e.g., protease inhibitors, salts, reducing agents, chelating agents, etc.)
- Flexible—lysate may be used for reporter assays, protein kinase assays, immunoassays and/or protein purifications
- Compatible—the lysate is compatible with standard protein assays such as Pierce Coomassie Plus (Bradford) Protein Assay
