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Thermo Scientific™ Zeba™ Spin Desalting Columns

Quickly remove salts and small peptides from large proteins (high MW). Thermo Scientific Zeba Spin Desalting Columns and Plates (40K MWCO) contain a size-exclusion chromatography resin that provides excellent protein desalting performance in a centrifuge format for 5μL to 4mL samples.Zeba Spin Desalting Columns and Plates are polypropylene devices containing a proprietary high-performance size-exclusion resin for effective re…

Thermo Fisher Scientific

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Average Rating 4.7

|4Scientists have reviewed this product

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Great column for desalting protein from 8M guanidine!

 

Average Rating 5.0

Application Area:

Protein desalting

Great product for desalting protein. Comes in multiple sizes to accommodate your sample size and different size beads to capture different matrices.

Review Date: 15 Jun 2021 | Thermo Fisher Scientific

Great results, saves lots of time!

 

Average Rating 3.7

Application Area:

Buffer exchange of stressed samples

Zeba spin desalting columns are an effective method of buffer exchange for stressed samples. They are easy to use and the recovery is reproducible though depending on the sample it can be kind of low at times. I would recommend them for use in buffer exchange as the process is much shorter than spin concentration.

Review Date: 18 Nov 2020 | Thermo Fisher Scientific

Great product!

 

Average Rating 5.0

Application Area:

Buffer exchanging antibodies

These Zeba spin columns are extremely easy to use. We use them to buffer exchange antibodies into more stable buffers.

Review Date: 26 Jul 2018 | Thermo Fisher Scientific

Average Rating 5.0

Application Area:

Sample Preparation

Extremely easy to use desalting/buffer exchange columns for small volumes. MWCO is 7kDa and a high yield is achieved.

Review Date: 22 Dec 2014 | Thermo Fisher Scientific

Quickly remove salts and small peptides from large proteins (high MW).

Thermo Scientific Zeba Spin Desalting Columns and Plates (40K MWCO) contain a size-exclusion chromatography resin that provides excellent protein desalting performance in a centrifuge format for 5μL to 4mL samples.

Zeba Spin Desalting Columns and Plates are polypropylene devices containing a proprietary high-performance size-exclusion resin for effective removal of salts and other small molecules (< 2000 daltons) while providing excellent recovery for protein larger than 40,000 daltons. Available in 5 different spin columns sizes, 96-well filter spin plates, Zeba Desalting Devices provide trouble-free desalting and buffer exchange for sample volumes ranging from 5µL to 4mL. The easy-to-use spin column format dramatically improves results over standard drip-column methodologies and the 96-well spin plate format enables more high-throughput processing.


Benefits:

  • High recovery – low-binding resin maximizes protein recovery
  • Fast – no screening of fractions or waiting for protein to emerge by gravity-flow
  • Easy-to-use – no cumbersome column preparation or equilibration
  • Flexible – available in spin columns and filter spin plates for a range of needs
  • Economical – comparable performance at lower cost than other commercially available resins

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