Products & ReviewLife Sciences

TOPO™ XL-2 Complete PCR Cloning Kit, with One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells

Thermo Fisher ScientificK8050-10Available: Worldwide

The TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb).

Request Pricing
Thermo Fisher Scientific

Receive your quote directly from the manufacturer.

Average Rating 5.0

|1Scientists have reviewed this product

Ease of Use
After Sales Service
Value for Money
Write your own review

Great company

 

Average Rating 5.0

Application Area:

Cloning

Very accurate and easy kit for cloning

Review Date: 24 Mar 2022 | Thermo Fisher Scientific

The TOPO™ XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb). The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™ SuperFi™ Green PCR Master Mix, which is included in the kit. Topoisomerase I activation of the vector allows PCR products to be ligated in just 5 minutes on your bench–top, resulting in high cloning efficiency (up to 90% positive recombinants with 10 kb fragments).

The pCR-XL-2-TOPO vector includes:

  • ccdB gene for positive selection
  • EcoR I site flanking the PCR product insertion site for easy excision of inserts
  • Ampicillin- and kanamycin-resistance genes for your choice of selection in E. coli
  • T7 promoter/priming site for in vitro RNA transcription and sequencing
  • T7, T3, and M13 forward and reverse primer sites for sequencing

The TOPO XL-2 Complete PCR Cloning Kit includes:

  • TOPO XL-2 PCR Cloning Kits containing the pCR-XL-2-TOPO vector
  • Platinum SuperFi Green PCR Master Mix—featuring a proofreading DNA polymerase (>300X fidelity compared to Taq) with high processivity and a density gradient to generate accurate, long PCR amplicons ready to load onto an agarose gel for gel extraction
  • PureLink™ Quick Gel Extraction and PCR Purification Combo Kit—designed to purify DNA fragments from agarose gels or direct PCR purification using a silica-based spin cartridge in < 30 minutes
  • One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli Cells—an improved chemically competent cell line, perfect for use in all cloning applications.

Product Overview

Links