Transcreener® ADP² TR-FRET Assay
The Transcreener ® ADP² TR-FRET Assay produces a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) signal that is less sensitive to interference from fluorescent compounds than other detection modes.
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Great product for HTS application.
Detection of ATPase activity
Transcreener is a competitive immunoassay for ADP detection that uses TR-FRET technology. Good for HTS application.
Review Date: 2 Feb 2021 | BellBrook Labs
The Transcreener® ADP² TR-FRET Assay produces a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) signal that is less sensitive to interference from fluorescent compounds than other detection modes. It is a simple, one-step homogeneous detection assay that accommodates a broad range of ATP concentrations (0.1 to 1,000 µM ATP) and produces asignal (Z´ > 0.7 at 10% conversion of 1 µM ATP). The assay can be used for virtually any ADP-producing enzyme including kinases and ATPases.
Displacement of tracer by ADP disrupts FRET from the antibody-bound Terbium chelate leading to a decrease in the TR-FRET signal. In addition to the time-resolved signal, the assay uses a far-red tracer to minimize interference from fluorescent compounds and light scattering.
- UNIVERSAL: detect any ADP-producing enzyme, using any substrate.
- SINGLE ADDITION: one-step, simple mix-and-read format.
- SENSITIVE: Z’ > 0.7 at low ATP conversion.
- LOW INTERFERENCE: far red, time-resolved signal.
