TriCat™ Urine ELISA

Dopamine, noradrenaline, and adrenaline are the catecholamines that are essential for the body's stress response and help regulate heart rate, blood pressure, and glucose metabolism. These compounds are produced in the adrenal glands and the brain, with their levels influenced by factors such as stress, diet, and certain medical conditions.

Adrenaline, noradrenaline, and dopamine are physiologically active molecules, which can be found in circulation in the human body and are excreted in urine.

The assay system is based on selective adsorption and followed by a solid phase enzyme-linked immunosorbent assay (ELISA) based on immunoglobulin binding. During the extraction step the catecholamines in standards, controls, and samples are selectively adsorbed onto the wells of the extraction plate and afterwards biotinylated by use of the acylation reagent. The biotinylated catecholamines are released and transferred onto the microtiter plate, coated with a goat anti-rabbit antibody. The extracted standards, controls, and samples are incubated in the coated wells together with antiserum containing rabbit antibodies specific to the targeted catecholamine and enzyme-conjugated streptavidin.

After the substrate reaction, the intensity of the developed color is proportional to the amount of the targeted catecholamine. Results of samples can be determined directly using the standard curve.

Regulatory status: EU: CE IVD. In the US for research use only. Not for use in diagnostic procedures.

Kit size: 3(AD, NAD, DOP) x 12 x 8

Method: ELISA

Incubation time: 1 x 2h, 1 x 1h, 1 x 25min

Standard range: AD: 3.5 - 113 ng/mL ; NAD: 12.6 - 375 ng/mL ; DA: 41.8 - 500 ng/mL

Specimen / Volumes: 25 µL urine

Substrate / isotope: PNPP 405 nm