Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution. Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages,…
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We regularly buy in bulk!
Routine mass spectrometry
Very easy to use - we find this trypsin/LysC to be incredibly reliable.
Review Date: 25 Oct 2019 | Promega Corp.
Always works perfectly. Never fails and is never contaminated.
Peptide mapping
In our lab we only use Promega Trypson/Lys-C. We've been using these reagents for over 9 years without ever having an issue attributable to the reagent. Not the same with all other protease suppliers.
Review Date: 27 Jun 2019 | Promega Corp.
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.
Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages, which occur at prominent quantities in trypsin digests. Trypsin/Lys-C Mix enhances digestion and compensates for the trypsin proteolytic inefficiency at lysine sites.
Trypsin/Lys-C Mix, Mass Spec Grade Features & Benefits:
- Simple to Use - Use standard overnight digestion with nondenaturing conditions.
- Enhanced Proteolysis - Increase peptide recovery, resulting in enhanced protein quantitation and improved reproducibility and eliminating the majority of missed cleavages (Figures 1 and 2).
- Tolerant to Trypsin-Inhibiting Contaminants - Generate mass spectrometry data from poor-quality sample material.
