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TSKgel® Butyl-NPR hydrophobic interaction chromatography column

TSKgel Butyl-NPR hydrophobic interaction columns for analysis of antibody-drug conjugates (ADCs) and as an orthogonal method to analyze protein aggregation, oxidation and deamidations.

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Tosoh Bioscience

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Average Rating 5.0

|2Scientists have reviewed this product

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Very good column, does exactly what you'd want it to.

 

Average Rating 5.0

Application Area:

Analyse protein following purification

Used for HIC. Very good resolution on peaks, clearer than the previous method and much faster than previous method (C-IEX). OCS was very clear and informative. Assays results appear better than with the previous column.

Review Date: 15 Aug 2023 | Tosoh Bioscience

Awesome yield, you must try!

 

Average Rating 5.0

Application Area:

Monoclonal Antibody

Our company uses Buthyl 650 M as a polishing 2 step. This resin is easy to use, package, and sanitize and is also convenient in terms of yield. We are also using different resins in development studies.

Review Date: 19 Jul 2022 | Tosoh Bioscience

TSKgel Butyl-NPR is based on a non-porous polymethacrylate base material with uniform 2.5 particle size. This makes fast analyses with high resolution possible and requires low sample loading. Hydrophobic interaction chromatography offers non-denaturing conditions for separating proteins as to their hydrophobic characteristics. Therefore it is used to determine the drug-antibody ratio (DAR) of antibody-drug conjugates (ADC). The Butyl-NPR column is well established for this purpose due to its cost-effectiveness and speed. Furthermore, it serves as a solution to determine protein aggregates, oxidations and deamidations.

Features:

  • Non-porous 2.5 µm polymethacrylate particles
  • Analyze drug-antibody ratio (DAR) in 15 min
  • High resolution and recovery

Column specifications:

  • Hydrophobic interaction chromatography
  • Particle size 2.5 µm
  • Non-porous
  • Inner diameter: 4.6 mm
  • Length: 3.5 cm or 10 cm

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