Ubiquitin reagent toolbox
A unique set of reagents for the investigation of ubiquitin.The HTRF® Ubiquitin toolbox assembles all the reagents for flexible assay development and HTS of each step of the ubiquitin system. Ubiquitin is directly labeled with the Eu3+-Cryptate. This conjugate is directly incorporated into the ubiquitin chain during the enzymatic step. Upon the addition of the XL665 conjugate, the HTRF® signal is proportional to the level of u…
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A unique set of reagents for the investigation of ubiquitin.
The HTRF® Ubiquitin toolbox assembles all the reagents for flexible assay development and HTS of each step of the ubiquitin system. Ubiquitin is directly labeled with the Eu3+-Cryptate. This conjugate is directly incorporated into the ubiquitin chain during the enzymatic step. Upon the addition of the XL665 conjugate, the HTRF® signal is proportional to the level of ubiquitination and remains stable for more than 24 hrs. HTRF® is particularly suitable for HTS of E2 and E3 activity. Miniaturization in a 1536-well format does not affect assay performances. The Cisbio team, experts in assay development for ubiquitination, propose this service applied to your specific targets.
