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Vimentin, bovine, 250 µg

PROGEN62011

Protein standard for immunoblotting, immunization and immunoassay.

PROGEN

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Protein standard for immunoblotting, immunization and immunoassay.

Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM sodium phosphate, pH 7.4).

For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

  • Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841
  • Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255

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