Wizard® Plus Minipreps DNA Purification Systems
The resin-based Wizard® Plus Minipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. When using the standard protocol, the entire miniprep process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Minipreps may be processed individually or in multiples with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-s…
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Not the easiest but easy enough and great yield
Plasmid purification from E coli cultures
This is my favourite miniprep kit. It's not the easiest to use, there is a 5-minute incubation step to degrade proteins and therefore more spins than some other kits, but the yield is much better and the kit is a bit cheaper per prep. Nice small box and the reps gave free reagent when I'd spilled a bottle and asked to buy another!
Review Date: 25 Feb 2016 | Promega Corp.
Food Analytics
It is Easy to use, adaptable for different sizes.
Review Date: 20 May 2014 | Promega Corp.
The resin-based Wizard® Plus Minipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA.
When using the standard protocol, the entire miniprep process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Minipreps may be processed individually or in multiples with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard® Minicolumn in Nuclease-Free Water (Cat.# P1193). The purified plasmid can be used directly for automated fluorescent DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions supplemented with a ribonuclease inhibitor, such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
Features & Benefits:
- High Performance: DNA is suitable for most molecular biology applications, including fluorescent sequencing.
- Confidence in Results: Purified DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector in BigDye® terminator sequencing.
- Fast: Entire procedure may be completed in 15 minutes or less.
- Convenient: No phenol extractions or ethanol precipitations required.
