Wizard® SV Gel and PCR Clean-Up System
The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests. Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, wh…
Purify PCR products for cloning
I used the Wizard SV Gel and PCR Clean-Up kit to purify PCR products I needed to make a MoClo basic part. The kit was easy to use, quick and gave me a high concentration of DNA. It was also very easy to purchase, we have a fridge containing the kit at UCL Bioscience stores.
Review Date: 27 Jun 2019 | Promega Corp.
Cloning
The Wizard PCR and gel purification kit is our go-to solution for fast a reliable purification of DNA for cloning applications. I have successfully used the purified DNA for In-fusion, Gibson and Golden Gate cloning applications.
Review Date: 10 Nov 2015 | Promega Corp.
Purify genomic DNA from mammalian cell lines
The product is versatile, efficient for both gel and column applications. The protocol is very straightforward to follow and results are significantly better than analogous products from other manufacturers.
Review Date: 16 Jul 2015 | Promega Corp.
Cloning
It's very easy to use!
Review Date: 21 May 2014 | Promega Corp.
The Wizard® SV Gel and PCR Clean-Up System is designed to extract and purify DNA fragments of 100bp to 10kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions such as restriction digests.
Up to 95% recovery is achieved depending upon the DNA fragment size. PCR products are commonly purified to remove excess nucleotides and primers. This membrane-based system, which can bind up to 40μg of DNA, allows recovery of isolated DNA fragments or PCR products in as little as 15 minutes, depending on the number of samples processed.
Wizard® SV Gel and PCR Clean-Up System Features & Benefits:
- Improved Productivity - Purify DNA fragments or PCR products in as little as 15 minutes.
- Enhanced Cloning Results - Up to 95% recovery eluted in as little as 15μl.
- Confidence in Results - Purified DNA routinely achieves 700 bases with >98% accuracy in automated fluorescent sequencing.
- Applications Tested - DNA is suitable for automated fluorescent sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.
- One System to Do It All - One system can replace up to four kits from other suppliers.