XL1-Blue MRF’ Supercompetent Cells
When DNA is methylated in a fashion unlike the bacterial host patterns, it is cleaved by the E. coli host restriction systems.
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When DNA is methylated in a fashion unlike the bacterial host patterns, it is cleaved by the E. coli host restriction systems. Cleavage of DNA before host replication creates libraries that lack complete representation. Our MR (Minus Restriction) series of competent cells are deficient in all known E. coli K12 restriction systems and make it possible to clone methylated DNA, which can be very useful in epigenetic studies where DNA methylation, instead of changes in DNA sequence, may be associated with heritable changes in gene expression or cellular phenotype.
- XL2-Blue MRF´ Ultracompetent Cells are high-efficiency derivatives of our XL1-Blue MRF´ Supercompetent Cells
- XL1-Blue MRF’ Cells are deficient in all known restriction systems and are are endonuclease (endA), and recombination (recA) deficient
- XL1-Blue MR Cells are restriction minus (McrA–, McrCB–, McrF–, Mrr, HsdR–) derivative of our XL1-Blue strain and is useful for cosmid-based cloning
For Research Use Only. Not for Use in Diagnostic Procedures.
