ZymoBIOMICS™ Spike-in Control I (High Microbial Load)
Whole cell spike-in control to assess absolute cell number in microbiome samples.
ZymoBIOMICS Spike-in Control I
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Great material. Excited to see more!
Identify sources of bias in wastewater surveillance DNA extraction
Zymo provided an effective material that was well quantified. When I reached out for more information they provided a response in a timely manner.
Review Date: 6 Dec 2022 | Zymo Research
Easy to use
Microbiome Sequencing
Useful product for microbiome sequencing library preprartion.
Review Date: 24 Nov 2021 | Zymo Research
Use it as SOP
Sequencing
Now part of our SOP
Review Date: 14 Jun 2021 | Zymo Research
Great kit - super helpful for our work!
Metagenomics/microbiome
We have been piloting spike in controls for quantitative metagenomics and really impressed by the technical support to modify protocols for our sample types. Also really straightforward use that will be beneficial for downstream success!
Review Date: 6 Jun 2021 | Zymo Research
Great product that delivered great results.
Metagenomics
The initial contact with Zymo was pleasant and very supportive. The product itself is easy to use, no additional optimization is required. I obtained high quality and reproducible results as promised. I used the product in combination with cell mock samples from Zymo.
Review Date: 4 Dec 2020 | Zymo Research
Easy to use, clear protocol, reproducible results.
Metagenomics
Easy to use, clear protocol, reproducible results. We had one question about an application for this spike, sent emails to several after care sales mail addresses, never got our answers.
Review Date: 28 Jan 2020 | Zymo Research
Want to continue using this product.
Normalizing libraries for NGS
Useful for our research, I will recommend this to others.
Review Date: 5 Jun 2019 | Zymo Research
Great improvment in skin microbiome study.
Skin microbiome
The ZymoBIOMICS Spike-in Control is a great product that allows us to go ahead with quantitative skin microbiome analysis. It is a smart product because with relatively short handling time, and relatively low costs we are obtaining a lot of information about the quality of our DNA extraction and the quantity of the input bacteria we are analyzing in every patient specimen. I´m so happy about it!
Review Date: 5 Jun 2019 | Zymo Research
ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can expose potential bias during DNA extraction. Moreover, with accurately quantified cell numbers, this standard enables absolute cell number quantification in microbiome measurements.
