Zymolyase® 20T (Arthrobacter luteus)

Zymolyase^® , produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls, to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase^® is Beta-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers at Beta-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit. There are two preparations of Zymolyase^® , Zymolyase^®-20T and Zymolyase^®-100T, having lytic activity of 20,000 units/g and 100,000 units/g respectively. Zymolyase^®-20T is ammonium sulfate precipitate while Zymolyase^®-100T is a further purified preparation by affinity chromatography. Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions.