Gene Fragments
Twist Bioscience Gene Fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.
Cloning of synthetic genes
I routinely order synthetic gene fragments from TWIST for our structural biology and assay programs. With the right end adapters they are extremely easy to clone into our suite of golden gate vectors for testing of recombinant protein expression (2-3 days turnaround upon receipt). Genes are delivered quickly and I have yet to encounter any problems.
Review Date: 5 Jun 2024 | Twist Bioscience
Synthetic Biology
I like the pricing and the quality of the gene synthesis. Customer support is also nice. I would strongly recommend Twist for gene synthesis.
Review Date: 13 May 2024 | Twist Bioscience
Cloning
Good price, good quality, fast turnaround time, I recommend it.
Review Date: 4 May 2024 | Twist Bioscience
protein expression
Twist had a super quick turnaround time compared to other Europe based gene synthesis companies for the offered price.
Review Date: 29 Mar 2024 | Twist Bioscience
Expression of recombinant proteins
At the beginning, I was worried about the mutation rate of the gene fragments compared to the clonal genes, but Andrew Chen (my primary contact) assured me of the quality of the fragments. I was not disappointed! Fragments performed as expected and with zero mutations!
Review Date: 27 Mar 2024 | Twist Bioscience
Synthesis of gene fragments for downstream cloning
My fragments came pretty quickly, within a week and a half, and it was good enough quality that I only had to do one round of cloning. The 4 colonies I sequenced were perfect! It expedited what would have been a long cloning process.
Review Date: 7 Mar 2024 | Twist Bioscience
For plasmid subcloning
I haven't had any issues with the gene I ordered. Additionally, the gene fragment synthesis of twist can tolerate higher sequence complexity compared to another website.
Review Date: 14 Feb 2024 | Twist Bioscience
Clone gene fragment into our construct.
We only needed a single correct plasmid with the gene fragment from Twist. So far, every colony I have picked has the gene fragment that we ordered, without mistakes. That, plus the fact that we got the gene fragment more quickly than we expected, means that we are more likely to use Twist for this service again.
Review Date: 12 Feb 2024 | Twist Bioscience
Gene & cell therapy
DNA fragment synthesized by Twist Bioscience is purchased by our lab. Its price and turnaround time are very favorable. The cloning result from synthesized DNA is very good and could achieve 100% accuracy.
Review Date: 6 Feb 2024 | Twist Bioscience
Generating oncogenic fusion proteins
Twist offers fast turnaround even overseas. The product quality is great. It is cheaper than other comparable services.
Review Date: 16 Jan 2024 | Twist Bioscience
Synthetic gene fragments are a simple and increasingly popular alternative to PCR-based cloning. Access to synthetic genes has been limited by cost, turnaround time, throughput, and quality. Twist Bioscience gene fragments improve your cloning process by minimizing colony screening. This allows you to save time and money by dramatically reducing cloning and sequencing costs. Think bigger, design on a grander scale, and accelerate your discoveries.
SPECS:
- 0.3 – 1.8 kb
- 100 ng – 1 μg per fragment
- No order limit