Drug discovery > Basic Research Products & Reviews

Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.

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X-gal, Galactopyranoside

Agilent Technologies

Agilent X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, BCIG) is a premium quality chromogenic substrate of β-Galactosidase. It is used with cloning systems that utilize β-galactosidase activity as an indicator of successful cloning.

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microRNA Spike-In Kit

Agilent Technologies

The Agilent miRNA Spike-in kit allows researchers to monitor the miRNA labeling and hybridization reactions. The Spike-in kit contains a labeling and hybridization spike-in, which correspond to complementary probe sequences on the microarrays. The Spike-ins and probes are designed using Drosophila.

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PAQ5000 2X PCR Master Mix

Agilent Technologies

The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.  Robust, cost-effective alternative to Taq  Equal to better yield with faster cycling conditions  Amplify up to 6 kb genomic DNA targets  Easily substituted…

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TaqPlus Precision PCR System

Agilent Technologies

The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.

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pBluescript II XR Library Construction Kit

Agilent Technologies

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.  New cDNA synt…

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LumiMax Superoxide Anion Detection Kit

Agilent Technologies

The LumiMax superoxide anion detection kit qualitatively detects the presence of superoxide anions and superoxide dismutase in cell cultures with a higher sensitivity than the standard luminol-based assay.  More sensitive than traditional assays (use as few as 10e4 cells)  Enhancer is non-cytotoxic and non-denaturing  Up to 40-fold signal enhancement for cultured cells  Proprietary enhancer which increases signal at lea…

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Mitochondrial Membrane Potential Detection Kit

Agilent Technologies

The Mitochondrial membrane potential detection kit uses a unique fluorescent cationic dye, JC-1, to signal the loss of mitochondrial membrane potential. In healthy non-apoptotic cells, the dye stains the mitochondria bright red.  Detect the loss in mitochondrial membrane potential - Early indicator of cellular apoptosis  Convenient kit format  Use with flow cytometer, fluorescence plate reader, fluorescence microscope

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pCMV-Script XR Library Construction Kit

Agilent Technologies

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.  CMV promoter for high-level protein expression in mammalian cells  Large multiple cloning site contains 15 unique sites  Clone PCR products generated with any PCR e…

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Oligo aCGH Prehybridization Buffer

Agilent Technologies

The Agilent Oligo aCGH Prehybridization Buffer is used to wet and condition arrays for hybridization on a Tecan HS 400 Pro or HS 4800 Pro with compatible hybridization chambers. It provides for better distribution of the sample over the active area, and helps prevent the occurrence of gradients in the signals.

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