Products, services, reviews and techniques used in the preliminary investigations of the disease; the cell biology, genetics and protein pathways using tissue culture, cloning and imaging.
The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression.
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays.
Method completed in less than one day versus 2 days with restriction enzymes
The pSG5 Vector is a 4.1 Kb eukaryotic expression vector constructed by combining pKCR2 and the Stratagene pBS vector. The high copy number of this plasmid allows for large quantities of double-stranded DNA to be obtained. Expression in vivo is achieved via transient expression in a variety of cell lines (highest level of expression is obtained following transfection of a cell line expressing the T antigen).
StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible. The resin is a non-toxic, non-combustible slurry of hydroxylated silica particles that is used to clean up DNA or concentrate dilute protein mixtures.
The StrataClone Mammalian Expression Vectors were designed for high-level expression of epitope-tagged proteins in mammalian cells. Use of StrataClone technology for the cloning steps allows rapid cloning of blunt-ended PCR products, produced using high-fidelity PCR enzymes, into the epitope tagging/expression vectors.
Our Vitality hrGFP (humanized, Renilla reniformis green fluorescent protein) products are optimal for mammalian expression protein studies that examine gene expression, transfection efficiency, and protein localization. Since hrGFP is derived from a different organism than EGFP, the hrGFP antibody does not cross react with the Aequorea victoria GFP variant-derived EGFP.
Restriction Enzymes Dpn I is an ideal enzyme for cloning and Southern blot analysis High purity enzyme preparation Includes 10X universal reaction buffer Glogos II Autorad Markers Luminescent peel-off stickers used for accurately marking and aligning autoradiographs. Easily affixed to plastic wrap or paper, these convenient markers emit low-level luminescence rather than radioactivity, making the Glogos II m…
Our Mycoplasma Plus PCR Primer Set detects infection by all forms of Mycoplasma with a robust single-band PCR product (triplet for Acholeplasma). This kit also includes controls to verify PCR amplification and Mycoplasma genomic DNA. The primer design permits higher annealing temperatures, reducing spurious bands from PCR contaminants.
The MycoSensor PCR & QPCR Assay Kits detect Mycoplasma infection of cell cultures by real-time quantitative PCR (qPCR) or PCR in less than two hours.
